{"id":7953,"date":"2010-08-18T00:00:49","date_gmt":"2010-08-18T00:00:49","guid":{"rendered":"http:\/\/cellgenuity.com\/?p=7953"},"modified":"2018-06-28T17:45:04","modified_gmt":"2018-06-28T17:45:04","slug":"amniotic-membrane-induces-epithelialization-in-massive-posttraumatic-wounds","status":"publish","type":"post","link":"https:\/\/cellgenuity.com\/index.php\/2010\/08\/18\/amniotic-membrane-induces-epithelialization-in-massive-posttraumatic-wounds\/","title":{"rendered":"Amniotic membrane induces epithelialization in massive posttraumatic wounds"},"content":{"rendered":"<div id=\"block-yui_3_17_2_6_1510095114584_67942\" class=\"sqs-block markdown-block sqs-block-markdown\" data-block-type=\"44\">\n<div class=\"sqs-block-content\">\n<p><strong>Amniotic membrane induces epithelialization in massive posttraumatic wounds\u00a0<\/strong><\/p>\n<p><a href=\"http:\/\/cellgenuity.com\/wp-content\/uploads\/2018\/05\/amniotic-membrane-induces-epithelialization-in-massive-posttraumatic-wounds.pdf\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-thumbnail wp-image-8031\" src=\"http:\/\/cellgenuity.com\/wp-content\/uploads\/2018\/05\/cellgenuitydownloadlogo-150x150.png\" alt=\"\" width=\"150\" height=\"150\" \/><\/a><\/p>\n<\/div>\n<\/div>\n<div id=\"block-yui_3_17_2_6_1510095114584_68005\" class=\"sqs-block html-block sqs-block-html\" data-block-type=\"2\">\n<div class=\"sqs-block-content\">\n<p>Wound Repair Regen. 2010 Jul-Aug;18(4):368-77. doi: 10.1111\/j.1524-475X.2010.00604.x.<\/p>\n<p>Insausti CL1, Alcaraz A, Garc\u00eda-Vizca\u00edno EM, Mrowiec A, L\u00f3pez-Mart\u00ednez MC, Blanquer M, Pi\u00f1ero A, Majado MJ, Moraleda JM, Castellanos G, Nicol\u00e1s FJ.<br \/>\n1Unidad de Terapia Celular, Hospital Universitario Virgen de la Arrixaca, El Palmar, Murcia, Spain.<\/p>\n<p><strong>Abstract<\/strong><br \/>\nLarge-surface or deep wounds often become senescent in the inflammatory or proliferation stages and cannot progress to reepithelialization. This failure makes intervention necessary to provide the final sealing epithelial layer. The best current treatment is autologous skin graft, although there are other choices such as allogenic or autologous skin substitutes and synthetic dressings. Amniotic membrane (AM) is a tissue of interest as a biological dressing due to its biological properties and immunologic characteristics. It has low immunogenicity and beneficial reepithelialization effects, with antiinflammatory, antifibrotic, antimicrobial, and nontumorigenic properties. These properties are related to its capacity to synthesize and release cytokines and growth factors. We report the use of AM as a wound dressing in two patients with large and deep traumatic wounds. Negative pressure wound therapy followed by AM application was capable of restoring skin integrity avoiding the need for skin graft reconstruction. AM induced the formation of a well-structured epidermis. To understand this effect, we designed some assays on human keratinocyte-derived HaCaT cells. AM treatment of HaCaT induced ERK1\/2 and SAP\/JNK kinases phosphorylation and c-jun expression, a gene critical for keratinocytes migration; however, it did not affect cell cycle distribution. These data suggest that AM substantially modifies the behavior of keratinocytes in chronic wounds, thereby allowing effective reepithelialization.<\/p>\n<p>PMID: 20636551 [PubMed \u2013 indexed for MEDLINE]\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Amniotic membrane induces epithelialization in massive posttraumatic wounds\u00a0 Wound Repair Regen. 2010 Jul-Aug;18(4):368-77. doi: 10.1111\/j.1524-475X.2010.00604.x. Insausti CL1, Alcaraz A, Garc\u00eda-Vizca\u00edno EM, Mrowiec A, L\u00f3pez-Mart\u00ednez MC, Blanquer M, Pi\u00f1ero A, Majado MJ, Moraleda JM, Castellanos G, Nicol\u00e1s FJ. 1Unidad de Terapia Celular, Hospital Universitario Virgen de la Arrixaca, El Palmar, Murcia, Spain. Abstract Large-surface or deep [&hellip;]<\/p>\n","protected":false},"author":14,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"pgc_meta":"","footnotes":""},"categories":[56,97,98,20],"tags":[],"class_list":["post-7953","post","type-post","status-publish","format-standard","hentry","category-amniotic-membrane","category-induces-epithelialization","category-massive-posttraumatic-wounds","category-wounds"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Amniotic membrane induces epithelialization in massive posttraumatic wounds - CellGenuity | Welcome to CellGenuity<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cellgenuity.com\/index.php\/2010\/08\/18\/amniotic-membrane-induces-epithelialization-in-massive-posttraumatic-wounds\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Amniotic membrane induces epithelialization in massive posttraumatic wounds - CellGenuity | Welcome to CellGenuity\" \/>\n<meta property=\"og:description\" content=\"Amniotic membrane induces epithelialization in massive posttraumatic wounds\u00a0 Wound Repair Regen. 2010 Jul-Aug;18(4):368-77. doi: 10.1111\/j.1524-475X.2010.00604.x. Insausti CL1, Alcaraz A, Garc\u00eda-Vizca\u00edno EM, Mrowiec A, L\u00f3pez-Mart\u00ednez MC, Blanquer M, Pi\u00f1ero A, Majado MJ, Moraleda JM, Castellanos G, Nicol\u00e1s FJ. 1Unidad de Terapia Celular, Hospital Universitario Virgen de la Arrixaca, El Palmar, Murcia, Spain. 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